Science

Lindis Biotech

Science | What we do

We combine leading scientific expertise in tumor immunotherapy and Triomab® antibody technology.

Mode-of-Action

Triomab® antibodies induce a highly potent immune response against the tumor

T-cells are redirected to and activated at the tumor cells in an MHC-independent manner by Triomab® antibody-mediated crosslink of tumor-associated antigens (TAA) with CD3 (signal 1).

Simultaneously or subsequently, accessory cells can be additionally bound via the Fc region of the Triomab® antibody. A process of mutual cross-talk between T-cells and accessory immune cells (macrophages, DCs and NK cells) is initiated via costimulatory molecules and cytokines.

Mode of Action, Triomab® antibodies induce a highly potent immune response against the tumor

[Source: Ruf & Lindhofer, Blood 98, 2526-34, 2001]

T-cells are strongly activated in a physiological manner (1), receiving a second co-stimulatory signal (signal 2), while accessory immune cells are stimulated via Fcγ-RI/IIa or III crosslinking.

As a result, tumor cells are effectively destroyed by a concerted attack of T-cells and accessory immune cells applying different killer mechanisms like perforin/granzyme-mediated lysis and apoptosis induction (2), or antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis (3).

Finally, T cell proliferation occurs as well as necrotic or apoptotic tumor particles are phagocytosed, processed, and presented by stimulated professional antigen-presenting cells (macrophages, DCs). This can lead to the induction of a patient-specific and long-term anti-tumor immunization effect (4).

Immunofluorescence staining of Triomab® antibody-induced immune cell complexes

Immunofluorescence staining of Triomab® antibody-induced immune cell complexes

Triomab® antibodies induce multiple immune cell complexes

As shown by the example (picture on the left side – desktop / above- mobile) Triomab® antibodies initiate the formation of multiple complexes between tumor cells and human immune effector cells:

EpCAM positive human tumor cells (HCT-8, stained in green by anti-cytokeratin 8, 18, and 19 FITC) are surrounded by and connected to human immune effector cells (PBMC, cell nucleus staining in blue) via EpCAM x CD3 bispecific Triomab® antibodies in red (texas red) functioning like a zipper between different cell types.

Advantages of Triomab® antibodies used in oncology

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Effective at low therapeutic dosages in the microgram (µg) range

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Full physiological activation of the patients’ most important cancer-fighting immune cells at the tumor site

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Linking innate and adaptive immunity resulting in vaccination-like effects against individual mutations of the patient’s cancer

Location

Lindis Biotech is located in the Innovation and Start-up Center for Biotechnology (IZB) in Martinsried, Munich – one of the most vibrant biotech hot spots in Europe.

Address

Lindis Biotech GmbH
Am Klopferspitz 19
82152 Martinsried